Pentr d topo manual - Installshield express bde merge module


Find and Purchase Gateway Entry Vectors - pENTR Products at Invitrogen Life Science Technologies. Kit Size Catalog no.

I am using a protocol from the manual of the TOPO. PENTR™ / D- TOPO® Cloning Kit ® with One Shot TOP10 Chemically Competent E.


Pentr/ d- topo Description: Gateway type ( lambda att- type) recombinational cloning system entry ( master) vector for TOPO cloning of insert; kanamycin resistance; recombinational cloning, TOPO cloning. I am having trouble cloning my PCR- amplified ~ 4. 4kb fragment into the pENTR/ D- TOPO vector ( 2. PENTR/ D- TOPO ® Cloning Kit.

Upon receipt, store the boxes as detailed below. Product Catalog no. 58kb) directional cloning vector from Invitrogen.

Pentr d topo manual. Product manuals are available for downloading from our Web site or by contacting Technical Service ( see page 52). 3 The Gateway ® Technology The Basis of Gateway ® The Gateway.

Pentr d topo manual. Shipping/ Storage Each pENTR™ 5′ - TOPO® TA Cloning® Kit is shipped on dry ice contains two boxes as described below. PENTR™ 1A VectorpENTR™ 2B Vector.
Glossary of Terms ® To help you understand the terminology used in the Gateway Technology page. Important Information pENTR™ Vectors This manual is supplied with the following products.

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User guide For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

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pENTR™ Directional TOPO® Cloning Kits Five- minute, directional TOPO® Cloning of. The pENTR™ / D- TOPO® Cloning Kit can be purchased with either TOP10 competent cells for standard cloning or Mach1™ - T1R competent cells for fast growth.

For Research Use Only. Not intended for use in animal or human therapeutic or diagnostic use.
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Features of the pENTR™ TOPO® Vectors The pENTR™ / D- TOPO®, pENTR™ / SD/ D- TOPO®, and pENTR™ / TEV/ D- TOPO® vectors are designed to facilitate rapid, directional TOPO® Cloning of blunt- end PCR products for entry into the Gateway® System. Features of the vectors include: 2! " attL1 and attL2 sites for site- specific recombination of the entry clone with a Gateway® destination vector!

vii Kit Contents and Storage Types of Kits This manual is supplied with the following kits.